FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001301146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences414791
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT233485.628858871094118TruSeq Adapter, Index 13 (97% over 38bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA5230.12608759592180158No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5200.12536434011345474No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA4640.11186356502431345No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)