FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001301182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284055
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN206817.28063227191917No Hit
ACACTCTTTCCCTACACGACGCTCTTCCGAGANNN3890.13694530988716974Illumina Single End PCR Primer 1 (96% over 30bp)
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA3390.11934308496593969No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA3260.11476650648641988No Hit
AGTGGGATTCCTGCTGTCAGTTAAGGAGATTTTGGGCTGAGACAATGGGG3130.11018992800690007No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA3070.10807766101635247No Hit

[OK]Adapter Content

Adapter graph