FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001301204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487990
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT85391.7498309391585893TruSeq Adapter, Index 7 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA5840.11967458349556342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)