FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001301212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences723606
Sequences flagged as poor quality0
Sequence length137-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT74741.0328825355234754TruSeq Adapter, Index 7 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA10270.14192806582587764No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)