FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001301274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences834265
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT623177.4696888878234144TruSeq Adapter, Index 7 (97% over 35bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT30220.3622350212462467TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT11950.14323985783893609TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)