FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001301278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819210
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT535456.536175095518853TruSeq Adapter, Index 7 (97% over 35bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT103411.2623136924598088TruSeq Adapter, Index 9 (97% over 35bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT9340.11401228012353365TruSeq Adapter, Index 9 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)