FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001301282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362818
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT468393.4369226118234426TruSeq Adapter, Index 19 (97% over 38bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT27230.19980657725389597TruSeq Adapter, Index 12 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA14120.10360884578865263No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)