FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001301284

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301284
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242304
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT429083.453905002318273TruSeq Adapter, Index 19 (97% over 38bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT21080.16968471485240327TruSeq Adapter, Index 12 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA12890.10375882231724279No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA12460.10029751172015866No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)