FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001301296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636914
Sequences flagged as poor quality0
Sequence length139-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT349545.488025070888691TruSeq Adapter, Index 7 (97% over 35bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT50320.7900595684817731TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT6380.10017050967634562TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)