FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001301313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1283117
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG150831.1754968564830799No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA30190.23528641581399046No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA27100.21120443420202523No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT25140.19592913194977543No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA25000.19483803893175758No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA21930.17091192775093775No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG20160.15711739459456933No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA19200.14963561389958982No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA17410.13568521031207598No Hit
AGTGGGATTCCTGCTGTCAGTTAATCCAGTCTATCATTGTTGGACATTTG15660.12204654758685295No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT14370.11199290477797426No Hit
AGTGGGATTCCTGCTGTCAGTTAACATTTTTTCCTTCATTTCAACTTTGG13230.10310829020268612No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)