FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001301320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809676
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT244651.351899456035224TruSeq Adapter, Index 7 (97% over 38bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA26680.147429705648967No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA21970.12140294726790872No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)