FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001301321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809676
Sequences flagged as poor quality0
Sequence length138-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA68550.378797088539606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60490.33425872918688204No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA58400.32270970052097725No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA44440.2455688200539765No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA40660.22468110313669407No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT35510.19622297029965582No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG35150.1942336639265813No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA34400.19008927564934275No Hit
AGTGGGATTCCTGCTGTCAGTTAATCCAGTCTATCATTGTTGGACATTTG32190.17787714485907974No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTTGGA21930.12118191322645601No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAAATGTGGCACATATACACCATGG19560.1080856462703821No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA18940.10465961862786488No Hit
AGTGGGATTCCTGCTGTCAGTTAACATTTTTTCCTTCATTTCAACTTTGG18100.10001790375735768No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)