FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001301325

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301325
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1370823
Sequences flagged as poor quality0
Sequence length137-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG167571.2224043512546843No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA21350.155745854862371No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA17950.1309432362894407No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT17580.12824412779768066No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA15900.11598871626752687No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA14960.10913152172089322No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT14870.10847498181749211No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA14100.10285791819950496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)