FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001301359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1289072
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139221.0800017376841635No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA28500.22108927972991424No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT22250.1726047885610734No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA18140.14072138716844365No Hit
AGTGGGATTCCTGCTGTCAGTTAAGGAGATTTTGGGCTGAGACAATGGGG15270.11845730882371194No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA13300.1031749972072933No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)