FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001301366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001301366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1504908
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT177011.17621808110529TruSeq Adapter, Index 7 (97% over 38bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA26650.1770872372264617No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA20440.13582225624423552No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA20310.13495841606264303No Hit
AGTGGGATTCCTGCTGTCAGTTAATCCAGTCTATCATTGTTGGACATTTG17590.11688422149393851No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA16330.10851161665696507No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA15610.1037272710358374No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT15080.10020546106472954No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)