FastQCFastQC Report
Mon 9 Jan 2023
EGAF00001302079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426407342
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG61228871.4359243842475864TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAACTCGTATG9884000.23179713448742637TruSeq Adapter, Index 6 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4509470.10575498017574003No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAAGCTCGTATG4280010.10037374075045828TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)