FastQCFastQC Report
Sat 24 Dec 2022
EGAF00001302119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369592115
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG116148393.142610063529088TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTATG17762040.4805849280631974TruSeq Adapter, Index 6 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10057540.2721253942335864No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAGCTCGTATG6874120.18599206316942124TruSeq Adapter, Index 6 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAACCTCGTATG4116470.1113787289536737TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)