FastQCFastQC Report
Sun 8 Jan 2023
EGAF00001302123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370342827
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG103667392.7992277004463215TruSeq Adapter, Index 8 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC13055410.35252228606009967No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTATG10914200.29470531637973374TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAGCTCGTATG7374360.19912252816496429TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTATG5675450.15324854664999357TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)