FastQCFastQC Report
Sat 24 Dec 2022
EGAF00001302127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences370143479
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG180426444.874500031378372TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTATG30470910.8232188794010876TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAGCTCGTATG23878610.6451176734090187TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTATG8129440.21962942645816544TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATATCGTATG4571120.12349589441233949TruSeq Adapter, Index 1 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC4255910.11498000752297463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)