FastQCFastQC Report
Sun 8 Jan 2023
EGAF00001302129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363059256
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG189783495.2273420072231955TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTATG29414990.8101980465690152TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAGCTCGTATG26149100.7202433092629925TruSeq Adapter, Index 1 (97% over 35bp)
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC13407240.3692851725559642No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTATG9011610.24821320076742515TruSeq Adapter, Index 1 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATATCGTATG5108730.14071339362850455TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)