FastQCFastQC Report
Fri 30 Dec 2022
EGAF00001302130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363059256
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC277094807.6322196837201695Illumina Single End PCR Primer 1 (96% over 33bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16957610.4670755453759868No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC10443670.287657450606355No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGGCTATAGTGTAGATCTC3678140.10130963304789012Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)