FastQCFastQC Report
Fri 30 Dec 2022
EGAF00001302159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381904730
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG90934822.381086508145631TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTATG8257450.21621753676630295TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAGCTCGTATG5438160.14239572261909403TruSeq Adapter, Index 8 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTATG4232940.11083759030688098TruSeq Adapter, Index 8 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)