FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001302175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1965758
Sequences flagged as poor quality0
Sequence length151
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC151505577.07230493275368TruSeq Adapter, Index 10 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCTTATCTCGTATGC58240.2962724811497651TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCTTATCTCGTATGC50950.2591875500443086TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTCATCTCGTATGC33610.17097730239429268TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTTATCTCGTATGC30840.15688604599345393TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCGTATGC26240.13348540359494912TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCTTATCTCGTATGC23390.11898717950022332TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTAATCTCGTATGC22740.11568056698739113TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCTTATCTCGTATGC21900.11140740620157721TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGGTTATCTCGTATGC21460.10916908388519848TruSeq Adapter, Index 10 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTGATCTCGTATGC21380.10876211619131144TruSeq Adapter, Index 10 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)