FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001302178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327876
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG34901726.283854817769132Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG769435.794441649672108Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG445193.352647385749874Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG371662.798905921938494Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG355142.674496715054719Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC180701.3608198355870578Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG163141.228578572095587Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG152841.1510110883847589Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG144101.0851916895854734Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG141721.0672683292717091Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCG140461.0577794914585399Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG104960.7904352514843253Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG103800.7816994960372806Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG102130.7691230205230006Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG86770.65344956908627Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG76860.5788191066033275Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG64840.48829860619515675Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG64050.4823492555027728Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG54730.41216197897996504Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGGCG51600.38859050092026665Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGGCG43810.3299253845991644Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG42370.3190809985269709Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG41510.3126044901782998Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGACG40400.30424527591431727Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGGCG29770.22419262039527788Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG29620.22306299684609104Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCC29340.2209543662209423Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCC28630.21560748142145802Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC28210.2124445354837349Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTGGCCG26340.19836189523720588Illumina Single End PCR Primer 1 (97% over 45bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGGCG26240.1976088128710813Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG24750.18638788561582556Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGTGGGCGCCG24550.1848817208835765Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGGCG24540.184806412646964Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG23480.17682373956604383Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGGCG23050.17358548539170826Illumina Single End PCR Primer 1 (97% over 47bp)
GAACGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG22530.16966945708786063Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG22500.16944353237802323Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGTGCCG21330.16063246869436604Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG20790.15656582391729348Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCC20780.15649051568068104Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG20530.15460780976536964Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG19300.14534489666203773Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGTGCCG19080.1436881154565637Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG17870.1345758188264567Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG16020.12064379505315255Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGACG15570.11725492440559208Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG14670.11047718311047115Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGGCG13870.10445252418147478Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCC13720.10332290063228795Illumina Single End PCR Primer 1 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)