FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001302180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2278020
Sequences flagged as poor quality0
Sequence length151
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG69134230.348372709633804Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG1442956.334228847859105Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG790843.4716113115775977Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG656642.882503226486159Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG641172.81459337494842Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC321181.409908604841046Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG309061.3567045065451577Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG288131.2648264721117461Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG281301.2348442946067197Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG279051.224967296160701Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCG263771.1578915022695149Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG258701.1356353324378188Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG217870.956400733970729Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGCCG180350.7916962976620047Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG168610.7401603146592216Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGCCG158130.6941554507862091Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGGGGCG106940.4694427616965611Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG91440.40140121684620855Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG80480.35328925997137867Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGGGGCG79550.34920676728035754Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGCCG77130.3385835067295283Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG67780.2975390909649608Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGACG63230.277565605218567Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGCGGCG49710.21821581900071116Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGCGGCG49370.2167232947910905Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCC49290.21637211262412093Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC48390.2124213132457134Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG48120.2112360734321911Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGGGGCG45950.20171025715314175Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCC44560.19560846700204565Illumina Single End PCR Primer 1 (97% over 49bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG41420.18182456694849036Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTGGCCG40880.17945408732144583Illumina Single End PCR Primer 1 (97% over 45bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGTGGGTGCCG40670.17853223413315072Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGGCG40620.17831274527879473Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGGAGATCTCGGGGGGTGCCG39240.17225485289856982Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGGGGGCGCCG37650.1652751073300498Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATATCGGTGGGCGCCG36960.1622461611399373Illumina Single End PCR Primer 1 (97% over 36bp)
GATCGGGAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG35910.15763689519846183Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGGCG35710.15675893978103791Illumina Single End PCR Primer 1 (97% over 47bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCC35330.15509082448793252Illumina Single End PCR Primer 1 (97% over 44bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGGGCCG34360.1508327407134266Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGGGGCG32780.14389689291577773Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGGGGCG31910.14007778684998376Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGGCG30550.13410769001150122Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGTGCCG28910.1269084555886252Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGGCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGGGCCG28600.12554762469161818Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGGGGGCGGCG26480.1162412972669248Illumina Single End PCR Primer 1 (97% over 41bp)
GATCGGAAGAGCGTCGTGGAGGGGAAGAGTGGAGATCTCGGTGGGCGCCG25820.11334404438942591Illumina Single End PCR Primer 1 (95% over 23bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGTCGCCG24690.10838359628098085Illumina Single End PCR Primer 1 (100% over 26bp)
GATCGGAAGAGCGGCGTGGAGGGGAAGAGTGTAGATCTCGGTGGGCGCCG23910.10495957015302763Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGGAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGCGCCC23140.10157944179594561Illumina Single End PCR Primer 1 (96% over 25bp)
GATCGGAAGAGCGTCGTGTAGGGGAAGAGTGTAGATCTCGGTGGGTGCCC22970.10083317969113528Illumina Single End PCR Primer 1 (97% over 44bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)