FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001302181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352129
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC39091.1101045355537318TruSeq Adapter, Index 10 (100% over 50bp)
TAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAA5020.14256139085392No Hit
TAACCTTTCTTTTCATAGAGCAGTTAGGAAACACTCTGTTTGTAAAGTCT4850.13773361466962392No Hit
TAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAA3870.10990290490132879No Hit
TAAACTCTGTGAGTTGAACGCACACATCACAAAGGAGTTTCTGAGAATCA3670.10422316821392161No Hit
TAATCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGTCTTTGCT3570.101383299870218No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)