FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948436
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC324093.4170993087567316TruSeq Adapter, Index 12 (100% over 50bp)
TAAAAAGTCAGGAAACAACAGGTGCTGGAGAGGATGTGGAGAAATAGGAA15960.1682770371432548No Hit
TAAGAAAATGTGGCACATATACACCATGGAATACTATGCAGCCATAAAAA11340.11956526323336523No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)