FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences785032
Sequences flagged as poor quality0
Sequence length138-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT235212.9961835950636404TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT49050.6248152941536141TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGGT22560.28737682030796197TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGTAT21630.2755301694707986TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGAT19290.2457224673643877TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGTAT18900.24075451701331918TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGGGGTT15220.19387744703400625TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGGAT14410.183559396304864TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGAT11660.14852897716271438TruSeq Adapter, Index 7 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA9280.11821174168696308No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA8410.1071293909038103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)