FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752294
Sequences flagged as poor quality0
Sequence length135-151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302204.01704652702268No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA22190.2949644686784688No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA21450.2851278888306965No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA17200.22863401808335568No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA16160.21480963559459468No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA13780.18317306797608382No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT12890.17134258680781717No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA12560.16695600390272952No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG12070.16044259292244786No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT11410.1516694271122726No Hit
AGTGGGATTCCTGCTGTCAGTTAATTTCCATTCCATTCGAGACCTTTCCA10550.14023772620810482No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA9940.13212919417142768No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA9210.122425541078355No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT8790.11684261738097075No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGG7560.10049262655291681No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)