FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences957681
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228152.382317285192042No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA25820.2696096090451831No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA24160.25227607105079874No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA22280.23264531717764056No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA18610.19432357956354987No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA14730.15380904497426595No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG14500.1514074101919115No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT14460.150989734577589No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA11210.11705359091388468No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT10980.11465195613153023No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA10660.11131055121695012No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA10630.11099729450620822No Hit
AGTGGGATTCCTGCTGTCAGTTAATTTCCATTCCATTCGAGACCTTTCCA9810.10243494441259668No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)