FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences968393
Sequences flagged as poor quality0
Sequence length137-151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207802.145823028460553No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA25580.26414895605399874No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA23630.2440125031882717No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA21260.21953896816684962No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA17950.1853586302255386No Hit
AGTGGGATTCCTGCTGTCAGTTAAATGGAAATGAATCAAATTGAATAGCA15930.16449933033386238No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT15310.15809697096116967No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG15160.15654801304842145No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA12070.12463948004580785No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT11290.11658489889951704No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCGATTGCAA11160.11524246870846856No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA10490.10832379003152645No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)