FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001302439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences642410
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT407956.350305879422799TruSeq Adapter, Index 7 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA18430.28688843573418843No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA15930.24797247863513955No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA11500.1790134026556249No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA8290.12904531374044612No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG8010.12468672654535266No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT7630.11877150106629722No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA7520.11705919895393907No Hit
AGTGGGATTCCTGCTGTCAGTTAATCCAGTCTATCATTGTTGGACATTTG7310.11379025855761896No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAAATGTGGCACATATACACCATGG7210.11223362027365702No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA6520.10149281611431951No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)