FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001302441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667088
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT394365.911663828460413TruSeq Adapter, Index 7 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA19910.2984613724126352No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA16020.24014822632096514No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA11630.17433981723550715No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCGCGTAT9000.13491473388818268TruSeq Adapter, Index 7 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA8720.13071738661166143No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG8050.1206737341999856No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT7350.11018036600868251No Hit
AGTGGGATTCCTGCTGTCAGTTAATCCAGTCTATCATTGTTGGACATTTG7350.11018036600868251No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA7310.10958074496917947No Hit
AGTGGGATTCCTGCTGTCAGTTAAGAAAATGTGGCACATATACACCATGG7090.10628282925191279No Hit
AGTGGGATTCCTGCTGTCAGTTAATGATTGCCATTCTAACTGGTGTGAGA6750.101186050416137No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)