FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences875464
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT449845.138303802326537TruSeq Adapter, Index 27 (97% over 39bp)
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG104501.1936527372913108No Hit
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA98491.1250034267542697No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGGCGGTAGTTGGAGCT27220.3109208374073634No Hit
CTGAATTAGCTGTATCGTCAAGGCACTTTTGCTTACGCTACCAGCTCCAA25470.29093143750057116No Hit
ATATGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG21320.2435280034358923No Hit
CTGTATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA19070.21782734641287363No Hit
GAAGCGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGACTG18110.206861732749719No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG15380.17567826889512303No Hit
GAAGATCCGCGACTGGTACCAGAAGCAGGGGCCCGGGCCCTCCCGTGACT15320.17499291804117587No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)