FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences935978
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT451204.8206261258277445TruSeq Adapter, Index 27 (97% over 39bp)
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA112021.196823002250053No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG101991.0896623638589797No Hit
ATTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG41130.4394334054860264No Hit
CTGAATTAGCTGTATCGTCAAGGCACTTTTGCTTACGCTACCAGCTCCAA28930.3090884614809322No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGGCGGTAGTTGGAGCT26060.278425347604324No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG15790.16870054638036364No Hit
CTGATTTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA15770.1684868661442897No Hit
GAAGCGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGACTG13880.14829408383530382No Hit
GAAGATCCGCGACTGGTACCAGAAGCAGGGGCCCGGGCCCTCCCGTGACT12960.13846479297590328No Hit
ATTAGGCCTGCTGAAAATGACTGAATATAAACTTGGCGGTAGTTGGAGCT10920.11666940889636294No Hit
TTCATGCTCAGCTGTGACTGCAGCTCAAGACCCTGGAGGGTGCGCCGCAG10720.11453260653562371No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)