FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917770
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT476085.18735630931497TruSeq Adapter, Index 27 (97% over 39bp)
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG113291.234405134183946No Hit
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA103741.1303485622759515No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGGCGGTAGTTGGAGCT29330.31957897948287695No Hit
CTGAATTAGCTGTATCGTCAAGGCACTTTTGCTTACGCTACCAGCTCCAA27040.2946271941771904No Hit
ATATGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG23900.2604138291728864No Hit
CTGTATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA18650.2032099545637796No Hit
GAAGCGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGACTG18400.2004859605347745No Hit
GAAGATCCGCGACTGGTACCAGAAGCAGGGGCCCGGGCCCTCCCGTGACT15970.17400873857284505No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG15340.16714427361975223No Hit
TTCATGCTCAGCTGTGACTGCAGCTCAAGACCCTGGAGGGTGCGCCGCAG9640.10503720975843622No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)