FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences952490
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT466904.901888733739987TruSeq Adapter, Index 27 (97% over 39bp)
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA120761.2678348329116316No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG109441.1489884408235258No Hit
ATTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG38240.4014740312234249No Hit
CTGAATTAGCTGTATCGTCAAGGCACTTTTGCTTACGCTACCAGCTCCAA31080.3263026383479092No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGGCGGTAGTTGGAGCT29270.3072998141712774No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG16390.17207529737845018No Hit
GAAGCGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGACTG15790.16577601864586505No Hit
GAAGATCCGCGACTGGTACCAGAAGCAGGGGCCCGGGCCCTCCCGTGACT13770.1445684469128285No Hit
TTCATGCTCAGCTGTGACTGCAGCTCAAGACCCTGGAGGGTGCGCCGCAG10770.11307205324990288No Hit
ATTAGGCCTGCTGAAAATGACTGAATATAAACTTGGCGGTAGTTGGAGCT10670.1120221734611387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)