FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735613
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT383235.209668670890808TruSeq Adapter, Index 27 (97% over 39bp)
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG26600.3616031799329267No Hit
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA23540.32000522013613136No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10850.14749603392000957No Hit
GAAGCGTCTCATGCTGGATCCCCACTTTTCCTCTTGCAGCAGCCAGACTG8880.120715648037759No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG7610.10345113531163805No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)