FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences782647
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT380654.863623063782267TruSeq Adapter, Index 27 (97% over 39bp)
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA25750.3290116744841544No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG24060.30741828691606815No Hit
ATTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG9720.12419392139751383No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG7910.10106727554056938No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)