FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences795125
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT399695.026756799245401TruSeq Adapter, Index 27 (97% over 39bp)
CTGAATTAGCTGTATCGTCAAGGCACTCTTGCCTACGCCACCAGCTCCAA28200.35466121678981294No Hit
ATAAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG25230.31730859927684324No Hit
ATTAGGCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTG9890.12438295865429963No Hit
CAGCCCAACCCTTGTCCTTACCAGAACGTTGTTTTCAGGAAGTCTGAAAG8130.10224807420216946No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)