FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046578
Sequences flagged as poor quality0
Sequence length139-151
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT90120.8610920542950453TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG42730.40828299467407114TruSeq Adapter, Index 6 (97% over 36bp)
AGTGGGATTCCTGCTGTCAGTTAAGCCGCTTTGGCCTGGTGGTTCAAGCG11570.11055076640250416No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)