FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences902192
Sequences flagged as poor quality0
Sequence length139-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT119211.3213373649954776TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG92941.0301576604536506TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT14320.15872452870342454TruSeq Adapter, Index 3 (97% over 34bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG14260.1580594817954493TruSeq Adapter, Index 3 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)