FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090748
Sequences flagged as poor quality0
Sequence length139-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT167591.5364685518561576TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG65020.5961046914594388TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT22910.21003934914389025TruSeq Adapter, Index 6 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT22310.20453853685727594TruSeq Adapter, Index 3 (97% over 34bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG21020.19271179044105513TruSeq Adapter, Index 3 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT12970.11890922559564629TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)