FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822185
Sequences flagged as poor quality0
Sequence length139-151
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT151291.840096815193661TruSeq Adapter, Index 2 (97% over 37bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT24400.29677019162353974TruSeq Adapter, Index 2 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAGCCGCTTTGGCCTGGTGGTTCAAGCG9350.1137213644131187No Hit
AGTGGGATTCCTGCTGTCAGTTAATTGAGAAGAGAGGATTTTCGGCTCAT9140.11116719473111282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)