FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001302577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603564
Sequences flagged as poor quality0
Sequence length139-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT208853.4602792744431414TruSeq Adapter, Index 7 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT37820.6266112624344726TruSeq Adapter, Index 2 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA6620.10968182330291402No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)