FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences603564
Sequences flagged as poor quality0
Sequence length139-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG147422.442491599896614No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA12360.20478358550211742No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTAGGA10900.18059393867096116No Hit
AGTGGGATTCCTGCTGTCAGTTAACCCGAATAGAATGGAATGGAATGGAA8310.1376821679225401No Hit
AGTGGGATTCCTGCTGTCAGTTAAAAAGTCAGGAAACAACAGGTGCTGGA7560.12525597948187764No Hit
AGTGGGATTCCTGCTGTCAGTTAAAGTAGTTTTTTCCAATTCTGTGAAGA7190.1191257265178175No Hit
AGTGGGATTCCTGCTGTCAGTTAAACTAAAGAGCTTCTGCACAGCAAAAG7040.11664048882968502No Hit
AGTGGGATTCCTGCTGTCAGTTAATTCCATTCCATTCCATTCCATTCCAT6520.10802499817749237No Hit
AGTGGGATTCCTGCTGTCAGTTAAGCTGATAAGCAACTTCAGCAAAGTCT6500.10769363315240803No Hit
AGTGGGATTCCTGCTGTCAGTTAACCTTTCTTTTCATAGAGCAGTTTGGA6250.10355157033885387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)