FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001302581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1222407
Sequences flagged as poor quality0
Sequence length136-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT309042.5281268840901596TruSeq Adapter, Index 7 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT14840.12139982837140167TruSeq Adapter, Index 2 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA12480.10209365620452107No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)