FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001302585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206957
Sequences flagged as poor quality0
Sequence length138-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT270352.239930668615369TruSeq Adapter, Index 7 (97% over 36bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT47370.3924746283421862TruSeq Adapter, Index 2 (97% over 35bp)
AGTGGGATTCCTGCTGTCAGTTAAACTCTGTGAGTTGAACGCACACATCA12220.10124635757529057No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)