FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001302651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742715
Sequences flagged as poor quality0
Sequence length32-151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC509036.853638340413213TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCCCGTATGC18830.25352928108359196TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGGATGC14150.19051722396881712TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGT12480.16803215230606625TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAATATCTCGTATGC10670.14366210457577938TruSeq Adapter, Index 6 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAAGC8260.111213587984624TruSeq Adapter, Index 6 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)