FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001302654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001302654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148237
Sequences flagged as poor quality0
Sequence length32-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGGAAGAGGGTAGATCTCGGGGGGCGCCG14330.12480001950816774Illumina Single End PCR Primer 1 (96% over 29bp)
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG13940.1214035081607717No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG13640.1187908071243132No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG12460.1085141830475764No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGATCTCGGGGGGCGCCG12030.10476931156198589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)